GC-MS and FTIR analysis of bioactive phytochemicals from ethanolic leaf extracts of Aloe secundiflora, Tephrosia vogelii & Nicotiana tabacum
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Journal of Research in Chemistry
Abstract
In the current study ethanol leave extracts of Aloe secundiflora, Nicotiana tabacum and Tephrosia
vogelii were examined using Fourier Transform Infrared Spectroscopy (FTIR) and Gas
Chromatography-Mass Spectrometry (GC-MS) to determine the key functional groups and
phytochemical constituents. The FTIR spectra indicated the occurrence of functional characteristic
peaks of primary and secondary amines, amides, alkanes, alkenes, aromatic and aliphatic amines in leaf
extracts of Nicotiana tabacum while for Aloe secundiflora it indicated the presence of characteristic
peaks associated with alcohols, phenols, alkanes, aldehydes, primary amines, aromatic and aliphatic
amines functional groups. The FTIR spectra of Tephrosia vogelii indicated the presence of functional
characteristic peaks of alcohols, phenols, alkanes, aldehydes, primary amines, nitro compounds,
aromatics, aliphatic and aromatic amines. Alkanes, aromatic and aliphatic amines were present in all
the three leaf extracts. The analysis using GC-MS for the ethanol leaf extracts of Aloe secundiflora
indicated the presence of 25 phytochemical constituents, Nicotiana tabacum indicated 15
phytochemical constituents and Tephrosia vogelii indicated 7 phytochemical constituents.
The two techniques complemented each other especially in detection of alkanes, alcohols and aromatic
amines since both methods showed their presence.